Cytofectins

Author: jppg

August undefined, 2024

WebA large number of liposome-based non-viral gene and siRNA delivery systems include monovalent cholesteryl cytofectins with acyclic head groups in their formulations. Progress in their clinical development has, however, been hampered by relatively low transfection efficiencies. WebCytotoxin (CTX) is a basic polypeptide that belongs to the three-finger toxin family. It is one of the significant toxins constituting approximately 40–60% of the cobra venom (Gasanov …

US5824812A - Polyfunctional cationic cytofectins, …

WebPromising advances in nonviral gene transfer have been made as a result of the production of cationic liposomes formulated with synthetic cationic lipids (cytofectins) that are able to transfect cells. However few cationic liposome systems have been examined for their ability to transfect CNS cells. WebIn addition to this first group of cytofectins (Group 1 lipids) comprised of traditional dialkenyl chains and the new TC series, a second group of cytofectins (Group 2 lipids) based on DODEG4, but capped with acyl chains of different lengths, were prepared to probe the effect of the length of the third chain. ... cianna wellington

Cationic liposome-mediated DNA transfection in ... - Gene Therapy

WebTypically, cytofectins are combined with a neutral zwitterionic lipid such as a phospholipid, because it has been found that the two amphiphilic lipid species in combination are able … WebApr 4, 2002 · Both of these cytofectins are predicted to produce relatively fluid bilayer liposome structures due to the short alkyl side chain of GAP-DLRIE (C12:0) and the mono-unsaturated C14 side chain of GAP-DMORIE (C14:1). In addition, the co-lipid used in the lipoplex formulations is also important, and thus structural differences in either or both ... WebFeb 15, 2002 · Anchor-dependent lipofection with non-glycerol based cytofectins containing single 2-hydroxyethyl head groups Detailed structure-activity investigations aimed at probing the anchor chain length dependency for glycerol-based lipofectins have been reported previously. dfw wholesale pools

Enhanced cationic liposome-mediated transfection using the DNA ... - PubMed

Websystems, including cytofectins, can substantially increase the delivery of antisense compounds to cells, as well as the con-sequent pharmacological effects (6,7). Thus it is … dfw wholesale flowers st louisWebSep 28, 2008 · Finally, the compounds have been introduced into man in a gene therapy clinical trial recently initiated by Nabel etal. Because there is now a rapidly growing list of compounds that act by a similar mechanism we are referring to this class of polynucleotide delivery reagents as the “Cytofectins”. dfw wifi login

"WebProvided is a collection of cytofectins (cytofectins are defined as chemical species that are cationic transfection amphiphiles) or cationic lipids that bind and transport … " - Cytofectins

Cytofectins

Novel cationic amphiphiles as delivery agents for antisense ...

WebHere, two homologous cationic cholesteryl cytofectins 3 beta[N-(N',N'-dimethylaminoethane)-carbamoyl] cholesterol (DC-Chol) and 3 beta[N-(N',N'-dimethylaminopropane)-carbamoyl] cholesterol (Chol-T) with 2 and 3 carbon spacer elements, respectively, have been formulated into liposomes with near equimolar … WebNational Center for Biotechnology Information

Did you know?

WebSep 17, 1996 · Amine containing compounds and their use in the generation of cytofectin:polynucleotide complexes for transfection of cells, formulations, counterions, and reaction conditions for maximizing the transfection include using cationic amine compounds that have the general structure: ##STR1## wherein R.sub.4 and R.sub.5 are a pair of … WebCefoxitin is an antibiotic used to treat a wide variety of bacterial infections. It may also be used before and during certain surgeries to help prevent infection. This medication is known as a ...

WebAug 1, 1999 · Cytofectin complexes. Complexes of oligonucleotides with Lipofectin® or Lipofectamine® were prepared as previously described ( 6 ), essentially according to the manufacturer's directions. Plasmid transfection assay For transfections, ECV304 or MDR3T3 cells were seeded in 6-well dishes ∼24 h prior to transfection and used at … WebSummary DNA, depending on base sequence, can induce a wide range of immune responses. While bacterial DNA is stimulatory, mammalian DNA is inactive alone and can, moreover, inhibit the response to ...

WebJul 21, 2008 · A range of monocationic and dicationic dioxyalkylglycerol cytofectins have been synthesised possessing methylene and short n-ethylene glycol spacers. The monocationic compounds were found to be effective in transfections when formulated as lipopolyplexes with peptide and DNA components, in particular with shorter PEG head … WebCompositions and methods of protecting aquatic invertebrates from disease is shown. In one embodiment, dsRNA or antisense RNA to a nucleic acid molecule of the disease-causing mic

WebThis invention relates to the field of biotechnology or genetic engineering. Specifically, this invention relates to the field of gene expression. More specifically, this inventio

WebFeb 1, 1996 · Cationic lipids (cytofectins) have gained widespread acceptance as pharmaceutical polynucleotide delivery agents for both cultured cell and in vivo transfection, and the cytofectins DOTAP and DC-Cholesterol are being tested in clinical human gene therapy trials. This study reports the effects of modifications in the hydrophobic domain … dfw wifi freeWebFeb 5, 1999 · The most promising advances in nonviral gene transfer have been in the production of synthetic cationic liposomes formulated with cationic lipids (cytofectins) able to transfect cells. Such... c. ian newberry m.dWebCationic lipids (cytofectins) have gained widespread acceptance as pharmaceutical polynucleotide delivery agents for both cultured cell and in vivo transfection, and the cytofectins DOTAP and... dfw wholesale nurseriesWebSep 28, 2008 · Cationic amphiphiles (cytofectins) are widely used for the transfection of cultured cells, and may become useful for the development of genetic medicines. … dfw wholesale glassWebVersion MV032206 Genlantis 858-457-1919 or 888-428-0558 (US Toll Free) 2 Purchaser Notification Limited License The purchase price paid for the Cytofectin™ Transfection … dfw wholesale securityWebFeb 1, 2008 · Two novel cholesterol-based cytofectins containing primary amino head groups, glycylcholesteryl formylhydrazide (MS10) and beta-alanylcholesterylformylhydrazide (MS11), have been prepared and incorporated into unilamellar cationic liposomes in equimolar amounts with dioleoylphosphatidylethanolamine (DOPE) as colipid. dfw wildlifeWebcytofectins transfective derivatized Prior art date 1996-04-09 Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.) Granted Application number CA002251169A Other languages French (fr) Other versions ... dfw wholesale properties