Phosphinothricin n-acetyltransferase
WebJun 1, 2014 · Phosphinothricin N-acetyltransferase (PAT) detoxifies the nonselective herbicide phosphinothricin (PPT, the ammonium salt of which is known as glufosinate), which is the active ingredient in ... WebMay 26, 2024 · PAT protein overexpression and purification. PAT (phosphinothricin acetyltransferase) expression condition were varied to obtain high yield, and transformed E. coli was examined in various condition such as temperature, IPTG concentration and culturing time. IPTG concentration of 0, 0.5 and 1 mM concentration were used at 18 °C, …
Phosphinothricin n-acetyltransferase
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WebBioCrick BioTech 88# Keyuan road,Hi-Tech Zone Chengdu, Sichuan 610042, PRC Email: [email protected] Tel:86-28-8543-3893 WebDroege, W.; Broer, I.; Puehler, A.: Transgenic plants containing the phosphinothricin-N-acetyltransferase gene metabolize the herbicide l-phosphinothricin (glufosinate) differently from untransformed plants. Planta, 187, 142–151 (1992) CAS Google Scholar
WebDroege, W.; Broer, I.; Puehler, A.: Transgenic plants containing the phosphinothricin-N-acetyltransferase gene metabolize the herbicide l-phosphinothricin (glufosinate) … WebResistance to bialaphos, a non-selective herbicide, was intro duced into cotton through genetic engineering. A gene encoding phosphinothric in acetyltransferase (bar) from Streptomyces hygroscopicus was inserted into elite varieties of cotton through particle bombardment. Based on the marker gene, β-glucuronidase (gus) expression, a total of 18 …
WebBiologically, phosphinothricin is a structural mimic of γ-glutamyl phosphate and an inhibitor of glutamine synthetase. 24 Phosphinothricin is first phosphorylated by Gln synthetase, and then the phosphorylated intermediate inactivates the enzyme. 24,25 Gln synthetase inhibition results in accumulation of glutamate and ammonia, with the latter … WebSep 10, 2016 · To examine the possibility of using the phosphinothricin N -acetyltransferase ( pat) gene as a selectable marker gene, we introduced the pat genes into D. salina with particle bombardment system under the condition of helium pressure of 900 psi from a distance of 3 cm. PCR analysis confirmed that the gene was stably inserted into the cells …
WebPhosphinothricin N-acetyltransferase (PAT) detoxifies the nonselective herbicide phosphinothricin (PPT, the ammonium salt of which is known as glufosinate), which is the active ingredient in...
Webphosphinothricin N-acetyltransferase (PAT) enzyme: eliminates herbicidal activity of glufosinate (phosphinothricin) herbicides by acetylation . Regulatory Approvals: Country, Year and Type of Approval. Country: Food direct use or processing: Feed direct use or processing: Cultivation sic143WebMar 20, 2006 · Similarly, bar and pat genes isolated from Streptomyces hygroscopicus and S. viridochromogenes, respectively, have been inserted into plants to encode phosphinothricin N -acetyltransferase to detoxify glufosinate. Glufosinate-tolerant crops have been commercialized using one of these two transgenes. Download to read the full … sic-146WebInactivates phosphinothricin (PPT) by transfer of an acetyl group from acetyl CoA. Can also acetylate demethylphosphinothricin but not PTT or glutamate. This enzyme is an effector … the perfume shop corkWebExpression of phosphinothricin N-acetyltransferase in Escherichia coli and Pseudomonas fluorescens: Influence of mRNA secondary structure, host, and other physiological conditions Protein Expr. Purif. 55 352-360 2007 Streptomyces viridochromogenes 17574436. brenda. 677277. the perfume shop crystal peaks tel numberWebNov 27, 2024 · In the 1980s, the bialaphos resistance ( BAR) gene and its closely related homologue the phosphinothricin acetyltransferase ( PAT) gene were isolated from Streptomyces hygroscopicus and... sic166Webl -Phosphinothricin ( l -Pt)-resistant plants were constructed by introducing a modified phosphinothricin- N -acetyl-transferase gene ( pat) via Agrobacterium -mediated gene … sic161WebGlufosinate (also known as phosphinothricin and often sold as an ammonium salt) is a naturally occurring broad-spectrum herbicide produced by several species of Streptomyces soil bacteria. Glufosinate is a non-selective, contact herbicide, with some systemic action. Plants may also metabolize bialaphos and phosalacine, other naturally occurring … the perfume shop crescent limerick